Skip to main content

Table 1 Experimental design and numbers of animals per group

From: Serine/threonine kinase-protein kinase B and extracellular signal-regulated kinase regulate ventilator-induced pulmonary fibrosis after bleomycin-induced acute lung injury: a prospective, controlled animal experiment

Mice

MIP-2, IP-10 (5 hours)

Hydroxyproline (5 hours)

Evans blue dye assay (5 hours)

Masson's trichrome stain (5 hours)

Immunohistochemistrya (5 hours)

Western blot assayb (1 hour)

RT-PCR (1 hour)

Control (without bleomycin)

6

6

6

 

6

6

6

Control (with bleomycin, 5 days)

6

6

6

6

6

6

6

6 ml/kg VT (with bleomycin, 5 days)

6

6

6

6

6

6

6

6 ml/kg VT (with bleomycin, 10 days)

 

6

 

6

   

30 ml/kg VT (with DMSO)

     

6

 

30 ml/kg VT (without DMSO)

   

6

   

30 ml/kg VT (with bleomycin, 5 days)

6

6

6

6

6

6

6

30 ml/kg VT (with bleomycin, 10 days)

 

6

 

6

   

30 ml/kg VT (with bleomycin, 5 days) + PD98059

6

6

6

6

6

6

6

VT 30 ml/kg (with bleomycin, 10 days) + PD98059

 

6

 

6

   

Control (with bleomycin, 5 days), Akt+/-

    

6

  

30 ml/kg VT (with bleomycin, 5 days), Akt+/-

6

6

6

6

6

6

6

30 ml/kg VT (with bleomycin, 10 days), Akt+/-

 

6

 

6

   

30 ml/kg VT (with bleomycin, 5 days) + SB203580

6

6

6

6

6

6

6

  1. Time points for measurements were determined according to our previous findings that mediator activation occurs early in ventilator-induced lung injury, and neutrophil infiltration occurs later [20]. aSerine/threonine kinase/protein kinase B, extracellular signal-regulated kinase 1/2, S100A4-positive. bJNK, p38, extracellular signal-regulated kinase, serine/threonine kinase/protein kinase B. Control, spontaneously breathing, nonventilated mice; DMSO, dimethyl sulfoxide; IP-10, 10 kDa IFNγ-inducible protein; MIP-2, macrophage inflammatory protein-2 (a functional homologue of IL-8); VT, tidal volume; 5 hours and 1 hour, time of mechanical ventilation.
Back to article page

Critical Care

ISSN: 1364-8535