Mice | MIP-2, IP-10 (5 hours) | Hydroxyproline (5 hours) | Evans blue dye assay (5 hours) | Masson's trichrome stain (5 hours) | Immunohistochemistrya (5 hours) | Western blot assayb (1 hour) | RT-PCR (1 hour) |
---|---|---|---|---|---|---|---|
Control (without bleomycin) | 6 | 6 | 6 | Â | 6 | 6 | 6 |
Control (with bleomycin, 5 days) | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
6 ml/kg VT (with bleomycin, 5 days) | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
6 ml/kg VT (with bleomycin, 10 days) | Â | 6 | Â | 6 | Â | Â | Â |
30 ml/kg VT (with DMSO) | Â | Â | Â | Â | Â | 6 | Â |
30 ml/kg VT (without DMSO) | Â | Â | Â | 6 | Â | Â | Â |
30 ml/kg VT (with bleomycin, 5 days) | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
30 ml/kg VT (with bleomycin, 10 days) | Â | 6 | Â | 6 | Â | Â | Â |
30 ml/kg VT (with bleomycin, 5 days) + PD98059 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
VT 30 ml/kg (with bleomycin, 10 days) + PD98059 | Â | 6 | Â | 6 | Â | Â | Â |
Control (with bleomycin, 5 days), Akt+/- | Â | Â | Â | Â | 6 | Â | Â |
30 ml/kg VT (with bleomycin, 5 days), Akt+/- | 6 | 6 | 6 | 6 | 6 | 6 | 6 |
30 ml/kg VT (with bleomycin, 10 days), Akt+/- | Â | 6 | Â | 6 | Â | Â | Â |
30 ml/kg VT (with bleomycin, 5 days) + SB203580 | 6 | 6 | 6 | 6 | 6 | 6 | 6 |