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Figure 5 | Critical Care

Figure 5

From: Serine/threonine kinase-protein kinase B and extracellular signal-regulated kinase regulate ventilator-induced pulmonary fibrosis after bleomycin-induced acute lung injury: a prospective, controlled animal experiment

Figure 5

PD98059, SB203580, and Akt-deficient mice reduced stretch-induced Akt and mitogen-activated protein kinase activation. After 5 days of bleomycin administration, wild-type mice or serine/threonine kinase-protein kinase B (Akt)+/- mice ventilated at a tidal volume of 6 ml/kg or 30 ml/kg for 1 hour were pretreated with 2 mg/kg PD98059 or 16 mg/kg SB203580 subcutaneously for 30 minutes. Western blot analysis was performed using an antibody that recognizes phosphorylated Akt expression, extracellular signal-regulated kinase (ERK) 1/2 expression, or P38 expression ((a) to (c) top panel), and an antibody that recognizes total Akt, ERK1/2, or P38 protein expressions in lung tissue ((a) to (c) middle panel). Arbitrary units expressed as relative Akt, ERK1/2, or P38 phosphorylation ((a) to (c) bottom panel) (n = 6 per group). *P < 0.05 versus control, nonventilated mice with bleomycin pretreatment; †P < 0.05 versus all other groups; ‡P < 0.05 versus control, nonventilated mice without bleomycin pretreatment.

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Critical Care

ISSN: 1364-8535