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Figure 2 | Critical Care

Figure 2

From: Circulating angiopoietin-1 and angiopoietin-2 in critically ill patients: development and clinical application of two new immunoassays

Figure 2

Comparison between detection of angiopoietin-1 and angiopoietin-2 concentrations in serum and plasma. (a) Angiopoietin-1 (Ang-1) and (b) angiopoietin-2 (Ang-2) concentrations were determined in parallel in serum and in ethylenediamine tetraacetic acid (EDTA) plasma samples obtained from the same five individuals. Importantly, the choice of anticoagulant matrix has a marked influence on Ang-1 measurement. Ang-1 was hardly detectable in EDTA-treated plasma, irrespective of the choice of buffer (calcium-supplemented buffer versus normal assay buffer; see Materials and methods). Ang-2 concentrations obtained from EDTA-treated plasma were lower (~80%) compared with values obtained from serum (100%). In contrast to Ang-1, the use of a calcium-supplemented buffer instead of the normal assay buffer could abolish the difference between serum and plasma for Ang-2.

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Critical Care

ISSN: 1364-8535