Figure 6
From: Oxidized phospholipids reduce ventilator-induced vascular leak and inflammation in vivo
Cells subjected to pathologic cyclic stretch (CS) (18%, 2 hours) were pretreated with vehicle (a) or OxPAPC (20 μg/mL) (b) followed by thrombin (0.5 U/mL) stimulation for 30 (c,d) or 50 (e,f) minutes. F-actin was visualized by immunofluorescence staining with Texas-Red phalloidin. Cells subjected to CS and thrombins (30 or 50 minutes) demonstrate barrier disruption, with the formation of transcellular actin stress fibers resulting in increased tension, cellular contraction, and paracellular gap formation (arrows). OxPAPC enhanced monolayer integrity and peripheral actin cytoskeletal rearrangement in ECs exposed to 18% CS alone and dramatically attenuated thrombin-induced gap formation and disruption of monolayer integrity and accelerated EC barrier recovery. Representative results from three independent experiments are shown. Two wells from each experiment were observed for each stimulation. OxPAPC, oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine; Thr, thrombin.