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Figure 7 | Critical Care

Figure 7

From: Platelet-derived exosomes induce endothelial cell apoptosis through peroxynitrite generation: experimental evidence for a novel mechanism of septic vascular dysfunction

Figure 7

Exosomes generate reactive nitrogen species. The graphic shows 4,5-diaminofluorescein diacetate (10 mM) fluorescence of exosomes incubated with L-arginine. The membrane-permeable superoxide dismutase mimetic Mn(III) tetrakis (4-benzoic acid) porphyrin chloride (SOD) had no inhibitory effect, whereas Nω-nitro-D-arginine methyl ester (L-NAME) and urate caused a significant decrease in fluorescent signals, suggesting the generation of reactive nitrogen species by exosomes, more importantly by septic exosomes and by exosomes induced with nitric oxide or lipopolysaccharide (LPS). Results are means ± SD of four experiments for each group. *P < 0.05 versus control, †P < 0.05 versus untreated. RFU, relative fluorescence units.

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Critical Care

ISSN: 1364-8535