Skip to main content
Figure 3 | Critical Care

Figure 3

From: The use of moderate hypothermia during cardiac surgery is associated with repression of tumour necrosis factor-α via inhibition of activating protein-1: an experimental study

Figure 3

Activation of p38 MAPK and c-Jun: hypothermia versus normothermia. Shown is activation of p38 MAPK (phospho-p38 MAPK) and c-Jun (phospho-c-Jun) in myocardium of pigs operated on under moderate hypothermia (28°C; circles) or under normothermia (37°C; squares). The time points of evaluation were as follows: 1 = before CPB; 2 = before aortic cross clamping; 3 = before removal of aortic clamp; and 4 = 6 hours after CPB. (a) In the upper panel, showing phospho-p38 MAPK findings, results are given as mean ± standard deviation. *P < 0.05. Band intensities for phospho-p38 MAPK were normalized for band intensities of total p38 MAPK. Lower panel: gel showing the effect of temperature on myocardial expression of phospho-p38 MAPK detected by Western blot. Results are representative of six independent experiments in each group. (b) In the upper panel, showing phospho-c-Jun findings, results are given as mean ± standard deviation. *P < 0.02. Band intensities for phospho-c-Jun were normalized for band intensities of actin. Lower panel: gel showing the effect of temperature on expression of phospho-c-Jun in the nuclear extract detected by Western blot. Results are representative of four independent experiments in each group (28°C = moderate hypothermia; 37°C = normothermia). CPB, cardiopulmonary bypass; MAPK, mitogen-activated protein kinase.

Back to article page

Critical Care

ISSN: 1364-8535