Figure 2From: New insights into the protein C pathway: potential implications for the biological activities of drotrecogin alfa (activated)Effect of thrombin on monolayer and cytoskeletal rearrangement of human primary endothelial cells derived from three different vascular beds. At early passages, cultured cells were plated in 8-well fibronectin-coated CultureSlides (Becton Dickinson, Bedford, MA, USA), 35,000 cells/well. After 24–48 hours, confluent monolayer cells were stimulated with 320 pM human thrombin (Sigma, St Louis, MO, USA) for 30 min at 37°C. Cells were fixed with 4% formaldehyde and stained for f-actin using Fluorescein isothiocyanate-conjugated phalloidin (Sigma, catalog number P5282). HCAEC, human coronary arterial endothelial cells; HMVEC-L, human lung microvascular endothelial cells; HUVEC, human umbilical venous endothelial cells. All cells were obtained from Cambrex (Walkersville, MD, USA). All images are shown at ×40 magnification.Back to article page