|
General principles that must be applied to design of animal model
|
Additional issues that must be considered to optimize the model
|
|
Proper randomization of animals
|
Models should be chosen on the basis of their relevance to the clinical situation, and not merely by the reproducibility of the model
|
|
Similar baseline characteristics of the experimental groups
|
Physiological parameters known to affect kidney function or susceptibility to injury should be controlled for, measured and reported (temperature, blood pressure, fluid status, type of anaesthesia, etc.)
|
|
Concurrent appropriate controls
|
Appropriate preparation of tissue for valid pathological interpretation
|
|
Blinded assessment of outcome
|
Fundamental requirements for a model should include morphology, haemodynamics and function
|
|
Consideration and reporting of mortality
|
Outcomes should be measures at multiple time points
|
|
Numbers of animals studied should be appropriate to reproducibility of outcome measure
|
Noninvasive biomarkers for renal parenchymal cell injury should be developed
|
| |
Models should be created that explicitly address comorbidities that are believed to predispose to acute renal failure and outcome in humans
|
| |
Experimental observations should be reproduced in other laboratories before they are generally accepted
|
