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Critical Care

Open Access

Additive effects of propofol and midazolam on dopamine vasodilatation of isolated rabbit renal arteries

  • C Eyigör1,
  • L Ýyilikçi1,
  • N Murat2,
  • H Akdad2,
  • S Gidener2 and
  • A Günerli1
Critical Care20037(Suppl 2):P098

Published: 3 March 2003


DopamineMidazolamRenal ArteryLeft Renal ArteryMidazolam Concentration

Background and objective

This study evaluated the effects of varying doses of propofol and midazolam on the vasodilatation due to low-dose dopamine in an isolated rabbit artery model.


Twenty-four New Zealand rabbits (2–2.5 kg) were used in the study. All of their left renal arteries were extracted. Renal artery strips 2–3 mm in length were mounted in 20 ml organ baths containing Krebs-Henseleit solution. In order to determine the maximal concentration for dilating the artery tissue, dopamine was added in cumulative log concentrations (10-9–10-5 M) to the bath after the tissue had been contracted with the KCl solution. The concentration that had the maximal relaxation effect was the one used in the subsequent trials of propofol and midazolam. After contraction with KCl solution and then relaxation with low-dose dopamine, tissue preparations were exposed to either cumulative log concentrations (10-9–10-5 M) of propofol (n = 8) or midazolam (n = 10). The force on the transducer was then recorded, and these measurements were translated into percentages of the initial contraction force.


Dopamine-induced relaxation (3 × 10-7 M) of the renal artery preparation was increased to a similar extent with the addition of midazolam at all concentrations (10-9–10-5 M). Propofol caused a concentration-dependent increase in relaxation (P < 0.05). Midazolam concentrations greater than 10-9 M (10-8–10-5 M) resulted in a significant increase in relaxation of the artery tissues (P < 0.05). The amount of relaxation in the propofol and midazolam groups was not significantly different (P > 0.05).


In this rabbit renal artery dilatation model using dopamine, the addition of either propofol or midazolam results in a statistically significant increase in the vasodilatatory effect of dopamine.

Authors’ Affiliations

Department of Anaesthesiology, Dokuz Eylül University, School of Medicine, Turkey
Department of Pharmacology, Dokuz Eylül University, School of Medicine, Turkey


  1. Hall RI, Sandham D, Cardinal P, et al.: Propofol vs midazolam for ICU sedation. Chest 2001, 119: 1151-1159. 10.1378/chest.119.4.1151View ArticlePubMedGoogle Scholar
  2. Nakamura K, Hatano Y, Hirakata H, et al.: Direct vasoconstrictor and vasodilator effects of propofol in isolated dog arteries. Br J Anaesth 1992, 68: 193-197.View ArticlePubMedGoogle Scholar


© BioMed Central Ltd 2003