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  • Meeting abstract
  • Open Access

Antibodies to ICAM-1 and PECAM-1 ameliorate pulmonary injury after intestinal ischemia-reperfusion in the rat

  • 1,
  • 1 and
  • 1
Critical Care19982 (Suppl 1) :P011

https://doi.org/10.1186/cc141

  • Published:

Keywords

  • Endothelial Cell
  • Pancreatitis
  • Acute Lung Injury
  • Mesenteric Artery
  • Superior Mesenteric Artery

Full text

Intestinal ischemia-reperfusion (I/R) gives rise to acute lung injury characterized by neutrophil sequestration and microvascular injury. Important mediators of I/R-associated injury include neutrophils and platelet-activating factor (PAF). During conditions of inflammation neutrophils and endothelial cells show an increased expression of adhesion molecules. ICAM-1 on endothelial cells is needed for high affinity bonds between neutrophils and endothelial cells, necessary for the further transmigration of neutrophils, where PECAM-1 is involved.

In the present study, a significant increase in albumin leakage over the pulmonary capillaries, as well as increased pulmonary MPO (myeloperoxidase)-content was found in rats subjected to 30 min intestinal ischemia (by clamping the superior mesenteric artery) followed by 12 h reperfusion. Treatment with anti-ICAM-1 or anti-PECAM-1 monoclonal antibodies significantly reduced the otherwise occuring increase in both albumin leakage and pulmonary MPO-content in pancreatitis animals. There was also an increase in serum IL-1β levels after intestinal I/R, which could be prevented by use of antibodies to ICAM-1 and PECAM-1.

In conclusion we found that the acute lung injury seen after intestinal I/R in the rat to a large extent could be prevented by blocking the adhesion/transmigration process of pulmonary leukocytes.

Authors’ Affiliations

(1)
Department of Surgery, Lund University Hospital, 221 85 Lund, Sweden

Copyright

© Current Science Ltd 1998

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