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Figure 4 | Critical Care

Figure 4

From: Modulation of chemokine gradients by apheresis redirects leukocyte trafficking to different compartments during sepsis, studies in a rat model

Figure 4

Effects of apheresis on leukocyte influx into the peritoneal cavity and lung. Eighteen hours after CLP, animals were randomly assigned to receive either apheresis by HA or sham treatment for 4 hours. Animals were then killed for sampling 48 hours after treatment. Cells were washed and centrifuged for three cycles and then stained with PE-Cy5 mouse anti-rat CD45 (OX-1), PE mouse anti-rat granulocyte (RP-1) and Biotin mouse anti-rat mononuclear followed by FITC streptavidin staining. Cells were gated on CD45. CD45 cells were analyzed for positive expression of either the granulocyte or mononuclear markers. Shown are (A) neutrophils; and (B) monocytes in the peritoneal cavity; (C) neutrophils and (D) monocytes in the lung. Comparisons are between apheresis (HA) and sham. Medians and ranges of cells per milliliter (natural log transformed, n = 14 to 18 each) are shown.

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Critical Care

ISSN: 1364-8535