Figure 1

Hyperglycemia alters diaphragm force-frequency curves. A) demonstrates representative force frequency curves (raw data from 1, 10, 20, 50 and 80 Hz stimulation frequencies) generated for intact diaphragm strips from a control animal treated with citrate buffer alone (left) and from a hyperglycemic animal two weeks after the initial injection of STZ (right). B) demonstrates mean data ± SEM (n = 6) for control (black) and two week hyperglycemic animals (red). As shown, hyperglycemia induced large reductions in the diaphragm specific force generation (force/cross sectional area) at all stimulation frequencies when compared to control animals. (*P = .002 for 1 Hz, P ≤0.001 for all other stimulation frequencies). SEM, standard error of the mean; STZ, streptozotocin.