Flow cytometric analysis of the frequency of CD38+ and Tim-3+ T cells. Peripheral whole blood was collected from 18 patients and 12 healthy controls (HC). Blood samples were stained with the fluorescent antibodies anti-CD3, anti-CD4/CD8 and anti-CD38 or anti-Tim-3 and were measured for the percentages of CD38+ and Tim-3+ T cells. Data are presented as flowcharts and expressed as the means of individual participants. The Mann–Whitney U test was used to analyze the differences between groups. (A) Representative flowcharts. FSC Lin, forward scatter, linear scale; SSC Lin, side scatter, linear scale. (B) Quantitative analysis of the percentages of CD38+ and Tim-3+ T cells. The horizontal lines indicate the median values for each group. FITC, fluorescein isothiocyanate; Tim-3, T-cell immunoglobulin mucin 3.