Proliferation and p60 NF-κB signalling. (A) Proliferating tubular epithelial cell (proliferating cell nuclear antigen-positive) count in the inner cortex at 24 hours post intervention. (B) p60 nuclear factor-kappa B (NF-κB) expression, and expression of proteins encoded by the NF-κB-dependent genes: (C) Inducible nitric oxide synthase (iNOS). (D) B-cell lymphoma protein 2 (Bcl2). (E) Vascular endothelial growth factor (VEGF). VEGF expression was increased in Sham + HF pigs, was also associated with increased microvascular density. Values derived from densitometry of western blots and expressed as a ratio to β-actin or glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Representative blots are shown. Values represent mean (standard error of the mean) of at least n = 4 per group. *P <0.05 versus sham, †P <0.05 versus Sham + HF, ‡P <0.05 versus cardiopulmonary bypass (CPB). HF, high-fat diet.