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Volume 17 Supplement 4

Sepsis 2013

  • Poster presentation
  • Open Access

Synaptic deficits in sepsis: role of glial cells

  • 1,
  • 2,
  • 1,
  • 1,
  • 2,
  • 1,
  • 1,
  • 2,
  • 1 and
  • 2
Critical Care201317 (Suppl 4) :P90

https://doi.org/10.1186/cc12989

  • Published:

Keywords

  • Recognition Memory
  • Recent Clinical Study
  • Cecal Ligation
  • Patch Clamp Experiment
  • Memory Dysfunction

Background

Recent clinical studies have shown that sepsis survivors can develop long-term cognitive impairment. The cellular and molecular mechanisms involved in these events are not yet completely understood. In this study, we investigate the synaptic deficits in sepsis and the involvement of glial cells in this process.

Materials and methods

Using a clinically relevant model of sepsis (cecal ligation and puncture), we observed a decrease of recognition memory 9 days after sepsis. At the same time, by colocalization between pre-synaptic and post-synaptic protein, synaptophysin and PSD-95, we observed a reduction of structural synapses in the hippocampus and cerebral cortex of septic mice. To define the molecular mechanisms accountable for synaptic loss in sepsis, we used an in vitro approach treating neuronal cultures with conditioned medium from astrocyte (ACM) and microglial (MCM) cultures stimulated with LPS.

Results

We observed that the MCM reduced the synapse number and the ACM increased the number of synapses. The analysis of conditioned medium composition showed that MCM had increased levels of IL-1β while the ACM had increased levels of TGF-β1, as compared with medium from the non-LPS-stimulated cultures. The increased levels of IL-1β, from microglial activated with LPS, accompanied by an increase of TGF-β1, from LPS-activated astrocytes, suggests an anti-synaptic activity in IL-1β and pro-synaptic actions in TGF-β1. Inhibition assays with the addition of soluble IL-1β receptor (IL-1 Ra) prevented the MCM-induced synapsis loss. To understand whether the loss in synapse density would have functional outcomes we performed patch clamp experiments in neurons treated with microglia conditioned medium (MCM) and MCM of LPS-stimulated cultures. Patch-clamp recordings in the MCM-treated neurons showed a reduction in postsynaptic current frequency, while an increase in current amplitudes suggests a functional synaptic deficit.

Conclusions

These findings show, for the first time, a correlation between synaptic deficits and memory dysfunction, suggesting a possible mechanism for cognitive impairment after sepsis as well as a glial-derived molecule mediating synapse reduction.

Authors’ Affiliations

(1)
Institute Oswaldo Cruz, FIOCRUZ,, Rio de Janeiro, Brazil
(2)
Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil

Copyright

© Moraes et al.; licensee BioMed Central Ltd. 2013

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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