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Figure 1 | Critical Care

Figure 1

From: The impact of crystalloidal and colloidal infusion preparations on coronary vascular integrity, interstitial oedema and cardiac performance in isolated hearts

Figure 1

Experimental protocols. Protocol 1: transudate formation (TF). Sample generation for assessing transudate formation and glycocalyx shedding in control (TF-Control) and study groups (TF-NaCl, TF-Ri-Ac, TF-6% HES, TF-10% HES, and TF-Gel). The same hearts were used to determine interstitial water content after the protocol, and in two additional cases in each group, for electron microscopy evaluation. Protocol 2: polymorphonuclear neutrophils (PMN). The intracoronary adhesion rates of PMN were determined in control (PMN-Control) and study groups (PMN-NaCl, PMN-Ri-Ac, PMN-6% HES, PMN-10% HES, and PMN-Gel) at the time indicated. Application and evaluation followed the detailed description given within the running text. Protocol 3: heart performance (HP). The determination of heart performance occurred in control (HP-Control) and study groups (HP-NaCl, HP-Ri-Ac, HP-6% HES, HP-10% HES, and HP-Gel) at the times indicated. For details, see Materials and methods.

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