Expression of aquaporin-1 in peripheral leukocytes of septic and nonseptic patients. Representative expression analysis of aquaporin-1 (Aqp1) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNAs in a nonseptic patient and a septic patient. End-point PCR was performed on RNA isolated from leukocytes of both patients at different time points (B, baseline; D, discharge; S, 6 to 12 hours from sepsis; SS, upon septic shock) and the end products were analyzed on a 2.5% agarose gel. (A) Expression of Aqp1 messenger RNA in peripheral leukocytes of one nonseptic patient and one septic patient. Lanes 1 and 2, nonseptic patient; lane 3, molecular weight standards; lanes 4 to 6, septic patient. (B) GAPDH was used as a positive control. Lanes 1 and 2, nonseptic patient; lanes 3 to 5, septic patient.