Figure 4
M1/M2 phenotyping of the macrophages in subcutaneous and visceral adipose tissue of septic mice. Gene expression of the M1-type macrophage marker tumor necrosis factor alpha (TNFα) (A), and inducible nitrous oxide synthase (iNOS) (B), Gene expression of the M2-type macrophage markers Arginase 1 (C), interleukin (IL)10 (D), and CD163 (E). Gene expression of the chemoattractant factor chemokine ligand 2 (CCL2) (F). AT is adipose tissue, A.U. is arbitrary units. Data boxes present median and IQR; whiskers represent the 10th and the 90th percentiles. White boxes present healthy controls (n = 8), light gray boxes are critically ill mice allocated to the total parenteral nutrition (TPN) protocol (cecal ligation and puncture (CLP)-fed) (n = 7), and dark gray boxes are critically ill mice allocated to the nutrient restriction protocol (CLP-restricted) (n = 6).