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Volume 16 Supplement 3

Sepsis 2012

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Development of a point-of-care-testing system for procalcitonin

Critical Care volume 16, Article number: P89 (2012) Cite this article

Background

Sepsis is a daily challenge in ICUs. Today various therapeutic strategies are known to improve survival in patients with sepsis. Early assessment is important for determination of the appropriate treatment. PCT has been proposed as a specific infection maker for this purpose.

Methods

A fluorescence dye-incorporated immunochromatographic assay (FICA) was developed for detection of the PCT concentration in serum. Serum mixed with fluorescence-conjugated detector was loaded onto a cartridge and incubated for 12 minutes. The fluorescence intensity of the cartridge was then measured in a laser fluorescence scanner. The analytical performance of the FICA system was evaluated by precision and recovery tests. The comparability of the new method was examined with an automated analyzer.

Results

A reliable correlation between area ratio (AT/AC), reflecting fluorescence intensity of test/control line, and PCT concentration was observed (r = 0.999). The CVs of intra-assay and inter-assay precision in a range of 0.26 to 2.09 ng/ml were 7.8% and 4.5%, respectively, and analytical recovery of the FICA system fell within 6.4% at the tested samples. When the FICA method was compared with Roche Elecsys analyzers, there were strong correlations (r = 0.993, n = 80).

Conclusion

The FICA system with point-of-care-testing appeared to be an easy, fast and suitable method for measurement of the PCT concentration in serum.

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Authors and Affiliations

  1. Boditech Med Inc., Chuncheon, Korea

    H Kim

  2. Hallym University, Chuncheon, Korea

    ES Yoon, S Ho & EY Choi

  3. Chonbuk National University, Jeonju, Korea

    SW Oh

Authors
  1. H Kim
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  2. ES Yoon
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  3. S Ho
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  4. SW Oh
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  5. EY Choi
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Kim, H., Yoon, E., Ho, S. et al. Development of a point-of-care-testing system for procalcitonin. Crit Care 16 (Suppl 3), P89 (2012). https://doi.org/10.1186/cc11776

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  • DOI: https://doi.org/10.1186/cc11776

Keywords

Critical Care

ISSN: 1364-8535