Skip to main content

Volume 16 Supplement 3

Sepsis 2012

Regulation of sepsis-induced IFNγ upon natural killer cell or natural killer T cell depletion in vivo


Natural killer (NK) and natural killer T (NKT) cells play a key role in bacterial infection and sepsis since they contribute to the bridging of innate and acquired immune responses. We have previously shown that in vivo depletion of these cell populations in a murine pneumococcal pneumonia sepsis model affected mortality.


Four groups of C57BL/6 mice (n = 5 to 15 mice/group) were infected intratracheally with 5 × 105 CFU Streptococcus pneumoniae. Twenty-four hours prior to bacterial inoculation, NK cell depletion was achieved by intravenous (i.v.) administration of anti-asialoGM1 rabbit polyclonal antibody in one group (NKDEPL), or anti-CD1d monoclonal antibody, clone 1B1 was given for NKT cell depletion in a second group (NKTDEPL). The control group received equal volume of isotype antibody control i.v. (C) and a fourth group received sham intratracheal installation of normal saline (S). All animals were euthanized 48 hours post infection. Serum and tissue samples were analyzed for bacterial colony counts, cytokine levels, splenocyte apoptosis rates and cell population analysis by flow cytometry. In parallel, specific miRNA expression analyses in splenocytes and lung histologic examination were also performed. Comparisons of numeric data between groups were made using the one-way ANOVA test for multiple groups.


We found that upon NK cell depletion there was a significant increase in the spleen NKT (CD3+/CD1d+) cell population compared with NKTDEPL, C and S (P = 0.014, P = 0.021 and P = 0.033, respectively). Interestingly, upon NKT cell depletion, spleen NK (CD3-/NK1.1+) cells increased significantly compared with NKDEPL, C and S (P < 0.0001, P < 0.0001 and P = 0.001, respectively). NKT depletion led to decreased lymphocyte apoptosis compared with C (P = 0.035), higher bacterial load in the lung compared with C and NKDEPL (P = 0.014 and P = 0.022 respectively) and in the liver compared with C (P = 0.012). In addition, serum levels of IFNγ were significantly increased and splenocytes from NKT depleted animals, incubated ex vivo in the presence or absence of IL-2, produced more IFNγ in comparison with all other groups. Furthermore, splenocyte miRNA analysis showed that miR-200c and miR-29a were downregulated, while miR-125a-5p was upregulated, in the NKT depleted animals compared with all other groups.


For the first time we have shown that NKT cell depletion resulted in an increase in spleen NK (CD3-/NK1.1+) cells and a higher IFNγ production, which were associated with specific changes in splenocyte miRNA expression.

Author information

Authors and Affiliations


Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Christaki, E., Diza, E., Giamarellos-Bourboulis, E. et al. Regulation of sepsis-induced IFNγ upon natural killer cell or natural killer T cell depletion in vivo. Crit Care 16 (Suppl 3), P46 (2012).

Download citation

  • Published:

  • DOI:


  • Natural Killer
  • miRNA Expression
  • Cell Depletion
  • Pneumococcal Pneumonia
  • Isotype Antibody Control