- Poster presentation
- Open access
- Published:
Procalcitonin, IL-10 and sCD25 as diagnostic and prognostic markers in critically ill patients
Critical Care volume 16, Article number: P43 (2012)
Background
Diagnostic and prognostic markers are of outmost importance in the critical patient referred to the ICU. The aim of the present study was the evaluation of procalcitonin (PCT), IL-10 and soluble CD25 (sCD25) as potential markers in the diagnosis and prognosis of cohorts of critical patients evaluated following a prospective design in a tertiary-center university hospital.
Methods
We studied 52 consecutive SIRS patients with suspected sepsis that were firstly stratified based on culture results (culture-positive and culture-negative), then subsequently divided into survivors and nonsurvivors. Venous blood samples were obtained from each patient within 6 hours of hospital/ICU admission (T-0) and at the end of first week of hospital/ICU stay (T-1). Serum samples were collected and used for simultaneous determination of IL-10 and sCD25, using a cytokine biochip array on the Evidence Investigator analyser (Randox Laboratories Ltd, Crumlin, UK), while PCT was assayed by an enzyme-linked fluorescent assay (VIDAS BRAHMS PCT; bioMerieux, France). Statistically significant differences between groups were established by the Mann-Whitney U test. The receiver-operating characteristic (ROC) curve was used to evaluate the diagnostic accuracy (defined by the area under the ROC curve (AUROCC)) of the analyzed cytokines and to determine the sensitivity and specificity at selected cutoff values. The statistical analyses were performed using SPSS 14.0 software (SPSS, Chicago, IL, USA).
Results
PCT, IL-10 and sCD25 were significantly (P < 0.05) increased in infectious versus non-infectious SIRS patients at hospital admission (T-0) and after 1 week of hospital stay (T-1). Diagnostic accuracy of PCT, IL-10 and sCD25 was evaluated by AUROCC and exhibited a significance index of 0.0001, 0.0021 and 0.0095 respectively at T-0, while at T-1 the P values were 0.0011, 0.0016 and 0.0201 respectively. Regarding prognostic markers, PCT showed a prognostic significance only at T-1 (P = 0.04). However when IL-10 and sCD25 values from survivors were compared with those from nonsurvivors, significant differences were found for the former and the latter marker with P = 0.0014 and P = 0.014 respectively at T-0, as well as with P = 0.0002 and P = 0.014 respectively at T-1.
Conclusion
In our study, well-known PCT and IL-10 markers and novel sCD25 significantly contributed to diagnosis and prognosis of SIRS and septic patients.
Author information
Authors and Affiliations
Rights and permissions
This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Focà, A., Liberto, M., Rametti, L. et al. Procalcitonin, IL-10 and sCD25 as diagnostic and prognostic markers in critically ill patients. Crit Care 16 (Suppl 3), P43 (2012). https://doi.org/10.1186/cc11730
Published:
DOI: https://doi.org/10.1186/cc11730