Figure 6

12/15-LO-deficiency influences acid-induced acute lung injury. Acid-induced ALI was initiated by intratracheal instillation of 2 µl/g HCL (pH 1.5) in WT and Alox15^-/- mice. (A) After two hours, the lungs were lavaged and the neutrophil count in the BAL fluid was determined (n ≥3 mice). (B) Wet/dry ratio of lungs from WT and Alox15^-/- mice after saline (SAL) or HCL instillation (n = 3). (C) Arterial blood was withdrawn from WT mice and Alox15^-/- mice two hours after saline (SAL) or HCL instillation and the paO₂/FiO₂ ratio was calculated (n = 3). Exemplary histological images of lungs from WT mice and Alox15^-/- mice after saline (SAL) or HCL instillation (D-G). Bar indicates 50 µm. ^#P < 0.05