Enhanced bacterial dissemination without NETs activity. Mice were treated with rhDNase (CLP + rhDNase; n = 44) or PBS (CLP; n = 44), respectively. Bacterial colony forming units (CFU) were recovered 6 hours (n = 8/8 in both groups), 24 hours (n = 7/14 and n = 10/14), and 48 hours (n = 9/22 and n = 11/22) after CLP from the peritoneal cavity, peripheral blood, lung, and liver. Data from two independent experiments are depicted. **P < 0.01, ***P < 0.001 versus adjacent time point; #P < 0.05, ###P < 0.001 versus CLP. CLP, cecal ligation and puncture; DNase, desoxyribonuclease; NETs, neutrophil extracellular traps; PBS, phosphate-buffered saline; rh, recombinant human.