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- Open Access
Moderate hypothermia during cardiac surgery provides hepatic protection by modifying the balance between gene expression of TNFα and IL10
Critical Care volume 5, Article number: P076 (2001)
To investigate the effect of moderate hypothermia during CPB on intrahepatic gene expression of TNFα, IL10, apoptosis regulatory proteins (Fas, Bak and Bcl-xL) and hepatic cell death during and after cardiac surgery.
Pigs were assigned to a temperature (T°) group during standardized cardiopulmonary bypass (CPB): normothermia (T°, 37°C; n = 8) and moderate hypothermia (T°, 28°C; n = 8). Liver probes were taken before and 6 hours after CPB for standard-and immunohistological examinations. Apoptotic cells were detected by TUNEL-staining. Intrahepatic gene expression of TNFα, IL10 and of apoptosis regulating proteins were examined by competitive RT-PCR.
Gene expression of cytokine and apoptosis regulating proteins was not detected before but 6 hours after CPB. Pigs operated on under 28°C showed lower TNFα-mRNA and higher IL10-mRNA than those operated on under 37°C (P < 0.05). While expression of apoptosis regulatory proteins and percentage of apoptotic hepatocytes were similar in both groups, percentage of necrotic hepatocytes was lower in 28°C than in 37°C group (P < 0.05). TNFα-mRNA after CPB was correlated with the percentage of necrotic hepatocytes (P < 0.05).
Moderate hypothermia during CPB provides hepatic protection by increasing IL10- and decreasing intrahepatic TNFα-gene expression without affecting gene expression of apptosis regulatory proteins.
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Qing, M., Vazquez-Jimenez, J., Klosterhalfen, B. et al. Moderate hypothermia during cardiac surgery provides hepatic protection by modifying the balance between gene expression of TNFα and IL10. Crit Care 5, P076 (2001). https://doi.org/10.1186/cc1143
- Gene Expression
- Apoptotic Cell
- Regulatory Protein
- Cardiopulmonary Bypass
- Hepatic Cell