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Mobilization of leukocytes in patients with severe sepsis and septic shock is associated with increased apoptosis, as detected by Annexin V binding
Critical Care volume 5, Article number: P064 (2001)
To clarify whether mobilization of leukocytes during human severe sepsis and septic shock occurs independently of apoptosis.
Over a 6 month period, 33 patients with severe sepsis or septic shock were studied in the intensive care unit. Annexin V binding by leukocytes was determined daily using flow cytometry and FITC-labeled Annexin V. Transient leukocytosis, or peaks in leukocyte counts, were defined as individual increase of at least 30% within 2 days, followed by a decrease of at least 30% within the following 2 days.
Nine, 14 and 10 peaks in neutrophil, monocyte and lymphocyte counts, respectively, were observed in 6, 9 and 10 patients; in all of these patients, increased Annexin V binding by neutrophils, monocytes and lymphocytes, respectively, by 69% up to 809% (median 215%), by 32% up to 973% (median 330%), and by 32% up to 4713% (median 224%), respectively, paralleled the neutrophil, monocyte and lymphocyte (in 13/14 episodes) peaks, respectively. During periods in which neutrophil, monocyte and lymphocyte numbers were stable, Annexin V binding was constant as well.
In conclusion, mobilization of leukocytes during severe sepsis and septic shock in critically ill patients is associated with increased apoptosis, as determined by Annexin V.
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Weiss, M., Barth, E., Fischer, G. et al. Mobilization of leukocytes in patients with severe sepsis and septic shock is associated with increased apoptosis, as detected by Annexin V binding. Crit Care 5, P064 (2001). https://doi.org/10.1186/cc1131
- Public Health
- Intensive Care Unit
- Flow Cytometry
- Septic Shock
- Emergency Medicine