Figure 2

Effect of γ-aminobutyric acid (GABA) on lung injury after high-tidal-volume ventilation. Adult female rats were anesthetized and intratracheally instilled with GABA (500 μM) and bicuculline (200 μM). Later, the animals were ventilated at a high tidal volume of 40 ml/kg BW for 60 minutes. At the end of ventilation, the left lungs were fixed for histopathology. Shown are the representative photomicrographs (×400) of lung tissue stained with hematoxylin and eosin. We observed marked alveolar edema (arrow) and septal thickening (arrowhead) after the instillation of saline. Similar changes were found when GABA and bicuculline were instilled. Scale bar, 40 μm.