Skip to main content

Amino acid concentrations in serum, urine and dialysate/ultrafiltrate solutions of continuous venovenous hemodiafiltration patients


A prospective study was performed for evaluating the amino acid losses during continuous venovenous hemodiafiltration (CVVHDF).


Serum, 24-hour urine and dialysate/ultrafiltrate solutions of CVVHDF were obtained on days 1, 3, and 5 from 11 critically ill patients (five males, six females, mean age 63.0 ± 18.1 (24 to 90)) in the surgical ICU. We analyzed 40 kinds of amino acid concentrations in serum (34 samples), urine (15 samples) and dialysate/ultrafiltrate solutions (30 samples) by high-performance liquid chromatography analysis. The mean dialysate amount was 918.2 ml (600 to 1,500 ml), mean replacement fluid amount 1,136.4 ml (1,000 to 2,000 ml) and mean blood flow rate 175 ml (100 to 200 ml), respectively. Nutritional support for CVVHDF patients was guided as protein intake at 1.2 to 1.5 g/kg/day, caloric intake at 30 kcal/kg/day.


Among the analyzed 40 amino acids, the five highest mean concentration levels of 24-hour dialysate/ultrafiltrate solutions were glutamine (65,178.3 μmol/l (hereafter, all units for amino acids are μmol/l)), alanine (48,633.3), glycine (33,959.5), proline (27,701.5), lysine (26,519.4); of serum were glutamine (694.4), alanine (438.1), glycine (349.7), lysine (275.7), proline (262.4); and of 24-hour urine were glycine (1,523.0), histidine (957.5), alanine (920.7), glutamine (904.6), lysine (699.1), respectively. Amino acid concentrations of 24-hour dialysate/ultrafiltrate solutions showed significant correlation with amino acid concentrations of serum (P = 0.000). The mean amount of total amino acid loss on day 5 of CVVHDF was 2.8 times that of day 1 and 1.7 times that of day 3. The increase of amino acid loss according to CVVHDF progression was most prominent in glutamic acid (8.9 times from day 1 to day 5).


The highest concentration level of 24-hour dialysate/ultrafiltrate solution was glutamine. The amount of amino acid loss after CVVHDF was correlated with the serum amino acid amount and increased according to CVVHDF progression.

Author information

Authors and Affiliations


Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Lee, J., Lee, Y. & Hong, J. Amino acid concentrations in serum, urine and dialysate/ultrafiltrate solutions of continuous venovenous hemodiafiltration patients. Crit Care 16, P364 (2012).

Download citation

  • Published:

  • DOI:


  • Proline
  • Lysine
  • Glutamine
  • Alanine
  • Glutamic Acid