Decreased peripheral CD4+/CD8+ lymphocytes and poor prognosis in aged sepsis
© Inoue et al.; licensee BioMed Central Ltd. 2012
Published: 20 March 2012
Aging is a significant factor and is associated with a poor prognosis in sepsis; however, the mechanism of immunological changes in aged sepsis is still unclear. The purpose of this study was to clarify the immunological changes in sepsis of aged patients.
Forty-four septic patients and 48 gender-matched healthy volunteers were prospectively enrolled in the study, which included the following investigations: (1) The SOFA score and clinical outcome were compared between adult sepsis (<65 years of age) and older adult sepsis (≥65 years of age). (2) Blood samples were collected from septic and control volunteers. Separated peripheral blood mononuclear cells were stained with CD4, CD8, programmed death-1 (PD-1), CD28, and CD62L antibodies and analyzed by flow cytometry, and serum was used to measure cytokine concentrations by using multiplex bead assay. Values were compared among four groups: normal adult (<65 years of age), normal older adult (≥65 years of age), adult sepsis (<65 years of age), and older adult sepsis (≥65 years of age) groups.
(1) No differences in SOFA scores were observed between adult sepsis (n = 19, 39 years) and older adult sepsis (n = 25, 78 years), but 3-month survival in older adult sepsis was significantly decreased compared with that in adult sepsis (36% vs. 4%, P < 0.05). (2) Population of CD8+ T cells in normal older adults was significantly less than that in normal adults (1.5 × 105 vs. 5.7 × 104/ml, P < 0.01), and percentage of PD-1+CD8+ T cells in the older adult sepsis group was significantly greater than that in the normal older adult group (40% vs. 29%, P < 0.01). Population of CD4+, CD62L+CD4+, and CD28+CD4+ T cells in the older adult sepsis group was significantly less than that in the normal older adult group (n = 26, 80 years) (1.8 × 105 vs. 5.9 × 104/ml, 1.6 × 105 vs. 5.4 × 104/ml, and 1.6 × 105 vs. 4.4 × 104/ml, respectively; P < 0.01); however, these values did not differ between the adult sepsis and normal adult (n = 22, 39 years) groups. Serum IL-12 level in older adult sepsis was increased when compared with that in the other three groups (P < 0.01).
Poor prognosis in older adult sepsis may be related to both preexisting decrease of CD8+ T cells with aging and loss of CD4+ T cells with sepsis.
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