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  • Poster presentation
  • Open Access

Decreased peripheral CD4+/CD8+ lymphocytes and poor prognosis in aged sepsis

  • 1,
  • 1,
  • 1,
  • 1 and
  • 1
Critical Care201216 (Suppl 1) :P5

https://doi.org/10.1186/cc10612

  • Published:

Keywords

  • Poor Prognosis
  • Peripheral Blood Mononuclear Cell
  • Septic Patient
  • Adult Group
  • Cytokine Concentration

Introduction

Aging is a significant factor and is associated with a poor prognosis in sepsis; however, the mechanism of immunological changes in aged sepsis is still unclear. The purpose of this study was to clarify the immunological changes in sepsis of aged patients.

Methods

Forty-four septic patients and 48 gender-matched healthy volunteers were prospectively enrolled in the study, which included the following investigations: (1) The SOFA score and clinical outcome were compared between adult sepsis (<65 years of age) and older adult sepsis (≥65 years of age). (2) Blood samples were collected from septic and control volunteers. Separated peripheral blood mononuclear cells were stained with CD4, CD8, programmed death-1 (PD-1), CD28, and CD62L antibodies and analyzed by flow cytometry, and serum was used to measure cytokine concentrations by using multiplex bead assay. Values were compared among four groups: normal adult (<65 years of age), normal older adult (≥65 years of age), adult sepsis (<65 years of age), and older adult sepsis (≥65 years of age) groups.

Results

(1) No differences in SOFA scores were observed between adult sepsis (n = 19, 39 years) and older adult sepsis (n = 25, 78 years), but 3-month survival in older adult sepsis was significantly decreased compared with that in adult sepsis (36% vs. 4%, P < 0.05). (2) Population of CD8+ T cells in normal older adults was significantly less than that in normal adults (1.5 × 105 vs. 5.7 × 104/ml, P < 0.01), and percentage of PD-1+CD8+ T cells in the older adult sepsis group was significantly greater than that in the normal older adult group (40% vs. 29%, P < 0.01). Population of CD4+, CD62L+CD4+, and CD28+CD4+ T cells in the older adult sepsis group was significantly less than that in the normal older adult group (n = 26, 80 years) (1.8 × 105 vs. 5.9 × 104/ml, 1.6 × 105 vs. 5.4 × 104/ml, and 1.6 × 105 vs. 4.4 × 104/ml, respectively; P < 0.01); however, these values did not differ between the adult sepsis and normal adult (n = 22, 39 years) groups. Serum IL-12 level in older adult sepsis was increased when compared with that in the other three groups (P < 0.01).

Conclusion

Poor prognosis in older adult sepsis may be related to both preexisting decrease of CD8+ T cells with aging and loss of CD4+ T cells with sepsis.

Authors’ Affiliations

(1)
Tokai University, Kanagawa, Japan

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