Skip to main content

Volume 15 Supplement 3

Sepsis 2011

Investigation into problems associated with the endotoxin activity assay

Introduction

Endotoxin activity assay (EAA) levels were compared with endotoxin levels determined by the turbidimetric kinetic method.

Methods

A specific method for the measurement of endotoxin, in the blood of patients under various conditions, and the influence of steroids on EAA levels and contamination of tubes used for the measurements were investigated.

Results

EAA levels increased in patients with injuries and acute pancreatitis. EAA levels did not increase in patients infected with Gram-positive bacteria. Endotoxin levels determined by the turbidimetric kinetic method did not increase in patients with injuries and acute pancreatitis and Gram-positive bacteria. When patients with long-term steroid use developed shock due to infection with Gram-negative bacteria, EAA levels did not increase but endotoxin levels determined by the turbidimetric kinetic method increased. EAA levels, but not endotoxin levels determined by the turbidimetric kinetic method, were suppressed by giving steroids in vitro. Endotoxin was detected in the tubes used for the measurements. This was suppressed by the addition of polymyxin B and anti-factor C antibody. EAA levels tended to increase immediately after direct hemoperfusion using a polymyxin-B-immobilized fiber column (PMX-DHP).

Conclusion

Our findings suggest that the EAA had limitations as a method to measure endotoxin.

Author information

Affiliations

Authors

Corresponding author

Correspondence to N Matsumoto.

Rights and permissions

This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Cite this article

Matsumoto, N., Takahashi, G., Kojika, M. et al. Investigation into problems associated with the endotoxin activity assay. Crit Care 15, P4 (2011). https://doi.org/10.1186/cc10373

Download citation

Keywords

  • Public Health
  • Steroid
  • Pancreatitis
  • Emergency Medicine
  • Acute Pancreatitis