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Figure 2 | Critical Care

Figure 2

From: Levosimendan inhibits release of reactive oxygen species in polymorphonuclear leukocytes in vitro and in patients with acute heart failure and septic shock: a prospective observational study

Figure 2

Scavenging capability of levosimendan. Oxidation of 2',7'dichlorodihydrofluoresceindiacetate (H2DCFDA) to fluorescent 2'-7' dichlorofluorescein (DCF) reflected by fluorescent activity (RFU) was measured in the presence of medium (i.e. control) or various concentrations of levosimendan (10 to 1000 ng/mL) with or without addition of hydrogen peroxide (H2O2). A scavenging activity of levosimendan would result in a reduction of RFU compared with control. The experiment without hydrogen peroxide was performed to exclude possible autofluorescence of levosimendan. Data are expressed as mean ± standard deviation. RFU, relative fluorescence units.

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Critical Care

ISSN: 1364-8535