Lung histopathology after sequential infection with C. albicans and P. aeruginosa in mice. A. Histological score of lung sections from BALB/c mice on Day 4. Peribranchiol and perivascular lung inflammation in mice was measured by two independent blinded examiners. Data are expressed as mean ±SE for each group. P < 0.05 for CaPa vs Pa mice. B. Immunofluorescence and periodic acid Schiff staining for C. albicans localization in lungs of BALB/c mice on Day 4. (a) Representative section of lung from mice challenged with both C. albicans and P. aeruginosa stained with fluorescent galenthus nivalis lectin (GNL) specific for terminal α-D-mannosyl, preferentially α-1,3 residues of C. albicans. The scale bars represent 10 μm. (b) Lung section from mouse receiving C. albicans and P. aeruginosae stained with PAS (periodic acid Schiff). The scale bars represent 5 μm.