Figure 3
From: Injurious mechanical ventilation affects neuronal activation in ventilated rats
Brain activation evidenced by c-fos immunotreactivity in the retrosplenial cortex. On the top (left): Coronal section diagram encompassing the area of interest. On the bottom: Representative images of RS from each experimental group after cresyl violet staining (left, a,c,e,g, 40X) and c-fos immunohistochemistry (right 100X and 400X). Brown dots represent c-fos staining. Black arrows indicate some examples of c-fos positive cells. HVt (h) and spontaneous breathing (d) increased the number of c-fos-positive neurons in the RS; lower levels of c-fos immunoreactive cells were found in unventilated (b) and LVt (f) animals. Data are presented as mean ± SE. *P < 0.05 respect to unventilated basal animals. Abbreviations: BAS, basal; LVt, low tidal volume; HVt, high tidal volume; Spont, spontaneous breathing; RS, retrosplenial cortex.