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Figure 8 | Critical Care

Figure 8

From: Endothelial dysfunction and renal fibrosis in endotoxemia-induced oliguric kidney injury: possible role of LPS-binding protein

Figure 8

LBP is pivotal in LPS-mediated endothelial dysfunction. (A) ELISA revealed the increased level of LBP in sera of endotoxemic pigs comparing with the control group at T0 and T9. A considerable reduction in serum LBP levels was found after 6 hours of CPFA treatment. (B) Plasma samples drawn from the CPFA circuit were also analyzed. A significant decrease of LBP was found in the plasma filtrate effluent from the sorbent cartridge 1 hour after circuit installation (T4 plasma postcartridge). After 6 hours from the start of treatment, LBP levels in plasma postcartridge (T9 plasma postcartridge) were lower than in plasma precartridge. The histograms represent the median ± IQR of at least five independent animals for each group. (C-E) Cultured ECs were treated with sera of healthy (CTR), endotoxemic (LPS), and CPFA-treated endotoxemic (LPS CPFA) pigs. (C, D) FACS analysis of ECs showed phenotypic changes after 12 hours of LPS sera incubation. In the presence of LPS CPFA sera, EC preserved their phenotypes. After LBP addition in LPS CPFA sera, ECs showed phenotypic changes (LBP + LPS CPFA). (D) Results are representative of three independent experiments. (E) The expression of collagen I was detected by real-time RT-PCR. The gene relative expression was normalized to the expression of GAPDH. The histograms represent the mean ± SEM and are representative of three independent experiments.

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Critical Care

ISSN: 1364-8535