Figure 2

Analysis of anions in canine serum using a polyvinyl alcohol (PVA) capillary. The retention times for some other organic anions not detected in canine blood serum are indicated. The peak at 6.283 minutes remains unidentified. The detection limits were as follows: acetate 1.0 mmol/L, α-ketoglutarate 10.0 μmol/L, citrate 0.1 mmol/L, fumarate 1.0 μmol/L, β-hydroxybutyrate 0.7 mmol/L, and urate 0.1 μmol/L. The conditions were as follows: PVA capillary (60 cm × 50 μm internal diameter); phosphate buffer, pH 7.0; running voltage, 20 kV; 25°C; detection: UV light absorption at 214 nm; sample: canine serum diluted with distilled water (1:100).