Figure 1

Analysis of anions in canine serum using a fused-silica capillary. As can be seen in the insert, the two anions, chloride and sulfate, migrated as distinct peaks, completely resolved from one another. The retention times for other inorganic and organic anions not detected in canine blood serum are indicated. The detection limits for sulfate and citrate were approximately 0.1 mmol/L. The conditions were as follows: fused-silica capillary (60 cm × 75 μm internal diameter); inorganic anion buffer, pH 7.7; running voltage, 20 kV; 25°C; detection: UV light transmission at 214 nm; sample: canine serum diluted with distilled water (1:100). Asterisk indicates unknown component introduced into serum from the 'coagulation' vial.