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Table 1 Effects of pretreatment on the rise of airway basal tone (g) in human bronchi at peak or plateau post-stretch (5 minutes, 2

From: Airway response to acute mechanical stress in a human bronchial model of stretch

  

Post-stretch peak

Post-stretch plateau

Pretreatment

n

Control

Pretreated

Control

Pretreated

Epithelium removal

12

1.43 ± 0.21

0.93 ± 0.15b

1.38 ± 0.22

0.89 ± 0.16b

MK476 (0.1 μM)

13

1.47 ± 0.19

0.89 ± 0.18a

1.45 ± 0.19

0.61 ± 0.18b

Indomethacin (1 μM)

9

1.25 ± 0.23

1.29 ± 0.23

1.21 ± 0.24

1.21 ± 0.21

1400 W (1 mM)

11

1.19 ± 0.11

0.89 ± 0.14a

1.13 ± 0.11

0.79 ± 0.16a

L-NAME (1 μM)

9

1.16 ± 0.12

0.97 ± 0.29

1.09 ± 0.11

0.91 ± 0.29

L-NAME (1 mM)

13

1.37 ± 0.20

0.86 ± 0.16b

1.31 ± 0.22

0.80 ± 0.16b

Nω-propyl-L-arginine (5 mM)

11

1.19 ± 0.11

0.83 ± 0.09a

1.13 ± 0.11

0.84 ± 0.10a

SR 140333 + SR 48968 +

SR 142801 (0.1 μM each)

9

1.01 ± 0.12

0.90 ± 0.16

0.96 ± 0.12

0.92 ± 0.17

BQ 123 + BQ 788 (0.1 μM each)

6

1.08 ± 0.16

1.20 ± 0.17

1.01 ± 0.14

1.17 ± 0.24

Atropine (0.1 μM)

9

1.23 ± 0.20

1.02 ± 0.22

1.16 ± 0.20

1.05 ± 0.22

Gadolinium (0.1 mM)

7

1.12 ± 0.11

0.98 ± 0.14

1.05 ± 0.12

0.95 ± 0.15

Mepyramine (1 μM)

9

1.15 ± 0.24

1.33 ± 0.20

1.11 ± 0.24

1.24 ± 0.23

Y27632 (1 μM)

7

1.12 ± 0.11

0.15 ± 0.07c

1.05 ± 0.12

0.11 ± 0.05c

  1. Values are means ± standard error of the mean. aP < 0.05; bP < 0.01; cP < 0.001 vs. paired control. 1400 W, selective inhibitor of inducible nitric oxide synthase; BQ 123, selective endothelin ETA-receptor antagonist; BQ 788, selective endothelin ETB-receptor antagonist; L-NAME, L-nitroarginine methyl ester; MK476, leukotriene Cyst-LT1 receptor antagonist; SR 140333, tachykinin NK1-receptor antagonist; SR 48968, tachykinin NK2-receptor antagonist; SR 142801, tachykinin NK3-receptor antagonist; Y27632, selective Rho-kinase (ROCK1 and ROCK2) inhibitor.