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Figure 4 | Critical Care

Figure 4

From: Post-operative infection and sepsis in humans is associated with deficient gene expression of γccytokines and their apoptosis mediators

Figure 4

Cytokine mRNA gene expression after in vitro stimulation of PBLs from septic ( n = 5) and control ( n = 5) patients. Interleukin (IL) -2, 7, Bcl-2 and Interferon gamma (IFN-γ) gene expression are expressed as normalised copy numbers of cytokine mRNA per 10 million β-Actin mRNA copy numbers is plotted on y axis. PBL's were incubated with medium alone, or stimulated with 1 μg/ml lipopolysaccharide (LPS), or stimulated with 3 μg/ml anti-CD3 monoclonal antibody (CD3) or alternatively stimulated with 1 μg/M PMA and ionomycin. Type of stimulation is plotted on × axis. Figure 1 a, b, c, d in vitro stimulation of PBLs from control and septic patients in absence of recombinant IL-2 (rIL-2) and Figure 1e, f, g, h in vitro stimulation of PBLs after pre-treatment of PBLs with rIL-2.

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Critical Care

ISSN: 1364-8535