The renal angiopoietin/Tie2 system in lethal human sepsis

Sepsis-induced multi-organ dysfunction syndrome (MODS) still has a high mortality. Improvements await a better understanding of the pathophysiological mechanisms. The angiopoietin (Ang)1/2 and Tie2 (tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 2) ligand/receptor system is an important regulator of endothelial cell responses to severe insults. Plasma Ang2 levels are prognostic in sepsis, but data on Ang/Tie responses in organs in humans are lacking.. We hypothesized that, in kidneys of patients who died of sepsis with acute kidney injury (AKI), the Ang/Tie signaling system is changed in such a way that microvessels become destabilized.

Sepsis-induced multi-organ dysfunction syndrome (MODS) still has a high mortality. Improvements await a better understanding of the pathophysiological mechanisms. The angiopoietin (Ang)1/2 and Tie2 (tyrosine kinase with immunoglobulin and epidermal growth factor homology domains 2) ligand/receptor system is an important regulator of endothelial cell responses to severe insults [1]. Plasma Ang2 levels are prognostic in sepsis, but data on Ang/Tie responses in organs in humans are lacking [2][3][4][5]. We hypothesized that, in kidneys of patients who died of sepsis with acute kidney injury (AKI), the Ang/Tie signaling system is changed in such a way that microvessels become destabilized.
Patients dying with sepsis-induced MODS were included. In the family conference preceding withdrawal or withholding of therapy, permission was asked for a renal biopsy as a partial autopsy to be performed immediately after death. The family was asked to give signed consent. The unaffected part of kidneys removed for renal cell carcinoma was used as a control (n = 8). mRNA was analyzed as described [3]. Histopathology revealed abnormalities without a recognizable pattern (Table 1). CD31 mRNA, a panendothelial marker, did not differ between patients and controls, suggesting equal endothelial content in the biopsies (Figure 1). Neutrophil gelatinase-associated lipocalin, a sensitive marker for renal damage, showed increased mRNA expression compatible with AKI and the histopathological findings. Ang1 mRNA in patients was decreased 10-fold, whereas Ang2 was decreased to a lesser extent. The Ang1/Ang2 ratio was decreased in sepsis. Tie2 mRNA was reduced as was the level of expression of Krüppel-like factor (KLF)2, a shear stress sensor. A correlation was found both in patients and in controls between KLF2 and Tie2 mRNA.
Our hypothesis that the Ang/Tie system is changed in a way that microvessels become destabilized in sepsisinduced AKI is supported by our finding that Ang1, Ang1/Ang2 ratio, and Tie2 mRNA levels were decreased in immediate post-mortem renal biopsies of patients with sepsis. The consequences on protein levels are the subject of further study. We previously reported flow sensitivity of Tie2 expression [5]. Combined with the KLF2 flow sensitivity, the correlation between KLF2 and Tie2 suggests that the decrease in Tie2 in patients dying Low expression of Tie2 is associated with low levels of KLF2 gene expression. Lines denote best fit, with the dashed lines representing the 95% confidence interval for best fit. ns, Not significant. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001. of sepsis might be due to changes in local blood flow. This mechanism would be additional to, and maybe synergistic with, other mechanisms such as inflammation and apoptosis as described in the literature and seen in the histology in our study.
Studies in organs of critically ill patients immediately after dying, especially examining gene expression status, are scarce. In biopsies taken immediately after circulation arrest, the effects of autolysis are avoided. However, patients dying of sepsis die after a long process with increasing organ damage. Instead of studying an illnessinduced change, we might be studying a shutdown process comparable to what happens at a cellular level in apoptosis. We show that immediate post-mortem samples contain high-quality RNA.
Our study is the first to show, on an organ level in humans, that the Ang/Tie2 axis is changed in AKI. The place of immediate post-mortem mRNA in the understanding of MODS should be further evaluated. It might be a new tool complementing human plasma and animal studies.