Lipid peroxidation in multidrug-resistant Gram-negative sepsis: translating science to the septic patient?

Multidrug-resistant Gram-negative induced sepsis poses an increasing threat to the vulnerable intensive care patient. The study by Toufekoula and colleagues reports the serum and tissue concentration of malondialdehyde (MDA), the toxic end product of lipid peroxidation, during the course of experimental and human Gram-negative sepsis. The complementary results from this dual experimental and clinical approach argue for highly compartmentalized lipid peroxidation during sepsis. Establishing a correlation between MDA concentration and survival provides valuable insights into the pathophysiology of Gram-negative sepsis. Yet, further studies are needed to understand and establish MDA as a biomarker during sepsis aggravated by organ failure.

Sepsis induced by MDR Gram-negative bacteria is a growing concern in intensive care units [2]. A vulnerable patient population, long-term use of invasive medical equipment and increasing use of broad-spectrum antibiotics considerably aff ect overall morbidity, mortality and health care costs [3]. Despite the amount of research in the fi eld of sepsis, a link between specifi c toxic mediators and patient outcome, which would enable biomarkers of organ failure to be defi ned and therapy adjusted accordingly, has not been established.
Th e study by Toufekoula and colleagues [1] features a dual approach to the organ distribution pattern of lipid peroxidation during sepsis. In vivo data from rats were compared to patient data from a historic study cohort [4]. Th is combined approach greatly enhances the value of the study since transferring results from rodent models to the clinical situation in septic patients is challenging [5]. Th us, comparing experimental and clinical data as presented by Toufekoula and colleagues is an excellent example of translational research.
In the rat sepsis model, one MDR P. aeruginosa isolate from one patient was investigated. However, in ventilatorassociated pneumonia diff erent strains of P. aeruginosa may diff er considerably with regard to onset and mortality [6,7]. Moreover, the pattern in which organs are aff ected diff ers according to the infectious agent [8]. Th us, the patterns of MDA production as reported by Toufekoula and colleagues might not be extrapolated to sepsis induced by other types of bacteria, which emphasizes the need to stratify both rodent and human sepsis for the underlying pathogen. Additionally, Toufekoula and colleagues induced sepsis in the rats by intraperitoneal injection, while sepsis in patients was caused by bacterial migration through the lung. Th is might explain why patients with pulmonary failure (that is, acute respiratory distress syndrome) showed high circulating MDA levels whereas pulmonary MDA concentrations in septic rats remained rather unchanged. Th is highlights the fact that the way organs are aff ected in

Abstract
Multidrug-resistant Gram-negative induced sepsis poses an increasing threat to the vulnerable intensive care patient. The study by Toufekoula and colleagues reports the serum and tissue concentration of malondialdehyde (MDA), the toxic end product of lipid peroxidation, during the course of experimental and human Gram-negative sepsis. The complementary results from this dual experimental and clinical approach argue for highly compartmentalized lipid peroxidation during sepsis. Establishing a correlation between MDA concentration and survival provides valuable insights into the pathophysiology of Gramnegative sepsis. Yet, further studies are needed to understand and establish MDA as a biomarker during sepsis aggravated by organ failure.
humans might diff er markedly from animal models [9] and may be directly dependent on the route of infection.
MDA is a biomarker for lipid peroxidation indicating oxidative stress [10]. Recently, circulating lipid peroxi dation products (other than MDA) during sepsis were shown to correlate with increased hospital mortality rates [11]. However, the present study shows that the con centrations of circulating MDA have to be interpreted with utmost caution in septic patients. Survivors among septic patients with hepatic dysfunction or with acute respiratory distress syndrome and concomitant cardiovascular failure showed higher circulating MDA concentrations than non-survivors. In contrast, survivors with acute renal dysfunction displayed lower circulating MDA levels than non-survivors. Questions concerning the underlying mechanism or the hypothesis that a toxic end product may predict survival under certain circumstances cannot be answered from this descriptive study. Microdialysis is an established method to monitor both metabolic and infl ammatory markers in clinical settings [12]. Several methods for MDA detection in microdialysis fl uid have been described [13] and it would be of great interest to evaluate MDA production directly in diff erent organs throughout the course of sepsis. Nevertheless, as an additional parameter, circulating MDA may potentially become a valuable tool in evaluating septic patients concordant with the recently proposed model of 'bioscore' combining diff erent biomarkers in one model [14].

Conclusions
Understanding the pathophysiology during MDR P. aeruginosa-induced ventilator-associated pneumonia and sepsis is desperately needed as infection rates are likely to increase in the future and novel therapeutic options will not be readily at hand [15]. Th e study by Toufekoula and colleagues provides valuable information about MDR P. aeruginosa-induced sepsis and, thus, a data foundation for MDA quantifi cation in vital organs. Although the data are entirely descriptive, the study sheds light on MDA as a promising biomarker, which may potentially facilitate the evaluation of intensive care patients during the course of severe sepsis.