LightCycler SeptiFast technology in patients with solid malignancies: clinical utility for rapid etiologic diagnosis of sepsis

We retrospectively evaluated 54 results of the SeptiFast test
from patients with solid malignancy admitted to the ICU between
June 2009 and August 2011. Specimens from suspected bloodstream
infection were analyzed using LightCycler SeptiFast (Roche
Molecular Diagnostics according to the manufacturers
instruction and evaluated in comparison with blood culture
results obtained from blood sampled no longer than 24 hours
before or after sampling for SeptiFast. Blood culturing and
identification were performed according to the routine
diagnostic procedures. The total number of blood cultures
analyzed was 85, and finding a microorganism by either of the
two tests was evaluated as positive. Consistently, negative
results from SeptiFast and blood culture were obtained in 21
(39%) cases.

We retrospectively evaluated 54 results of the SeptiFast test from patients with solid malignancy admitted to the ICU between June 2009 and August 2011. Specimens from suspected bloodstream infection were analyzed using LightCycler SeptiFast (Roche Molecular Diagnostics, Prague, Czech Republic) according to the manufacturer's instructions and evaluated in comparison with blood culture results obtained from blood sampled no longer than 24 hours before or after sampling for SeptiFast. Blood culturing and identifi cation were performed according to the routine diagnostic procedures. Th e total number of complementary blood cultures analyzed was 85. Consistently, negative results from SeptiFast and blood culture were obtained in 21 (39%) cases.
To assess the true positivity of both microbiological methods, discrepant cases were evaluated in the context of clinical and laboratory fi ndings by two independent physicians experienced in critical care ( Figure 1). Th e clinically relevant presence of a pathogen detected by blood culture but not by SeptiFast was recorded for Klebsiella pneumoniae/oxytoca, and in both cases the presence of another member of Enterobacteriaceae was reported by SeptiFast, Escherichia coli and Enterobacter cloacae/aerogenes -thus misidentifi cation of strains with atypical phenotype cannot be excluded [3]. Our results also show that SeptiFast is more effi cient in detection of clinically relevant infection by E. coli and Pseudomonas aeruginosa.
Th e turnaround time for blood culture analysis is 24 to 48 hours. Th e workfl ow in our laboratory allows SeptiFast analysis every working day during a day shift. In such conditions, the turnaround time of SeptiFast results for samples delivered to the laboratory on a workday between 7 am and 2 pm was as follows: 100% of samples, 10 hours; 97% of samples, 8 hours; 59% of samples, 6 hours. Th e average turn around time for tests performed with manual DNA isolation was 6 hours 11 minutes; by implementation of automated isolation of DNA using the MagNA Pure Compact System [4], the mean turnaround time was shortened to 5 hours 17 minutes.
In conclusion, detection of pathogen by the LightCycler SeptiFast system is generally not inferior to the results from blood culture. Th e added value of multiplex DNA amplifi cation-based pathogen detection is the shorter test turnaround time and probably the increased true sensitivity of detection of certain pathogens. Moreover, in clinical settings pathogen detection may be required after an antibiotic administration; in such conditions, cultivation independent methods are of clear benefi t [5].

Competing interests
Reimbursement from Roche Diagnostics unrelated to the present work: in 2008, DV and LD for a lecture on a pharmacogenomic and predictive oncology topic, respectively, and in 2010, MV and LD for an expertise work on a protocol for automated DNA isolation from formalin-fi xed paraffi nembedded tissues.   [2], nine were detected in the present study and all pathogens detected by blood culture were on the SF menu, 18 microorganisms were detected by both BC and SF, 12 by BC only, and 18 by SF only. The clinical evaluation is as follows: each pathogen detected by either method was classifi ed as possible infection (based on clinical data; for example, benefi t from antimicrobial therapy), as probable infection (based on clinical data and cultivation of the pathogen from other site), as highly probable infection (based on clinical data and presence of the pathogen in blood within the relevant time window), as unlike infection (for example, contamination) or as undetermined. To obtain orientation in false positive and false negative results in pathogen detection by SF and cultivation, we assigned a score value to each positive classifi cation as follows: possible infection (score 2), probable infection (score 3), and highly probable infection (score 4). Black boxes represent summary of cases when pathogen was detected by both methods, SF and BC. Red boxes left of concordant events show detection by BC only, and blue objects right of black squares represent events of pathogen detection by SF only. Empty boxes, cases evaluated by both ICU experts as unlike infection; unclear, cases evaluated by ICU experts discrepantly (for example, unlike vs. possible or probable infection). The size of fi lled objects together with abbreviated classifi cation describe the evaluation of ICU experts for each case: h.pr., highly probable infection; pr., probable infection; po., possible infection. Coagulase-negative staphylococci (CoNS) were detected far more often by BC than SF, probably as a result of contamination of blood culture sample by staphylococci from normal skin fl ora. Regarding CoNS, SF is able to refl ect the amount of CoNS DNA template; if its level is below the discrimination threshold (crossing point (CP) value of amplifi cation growth curve >20), the result is reported as negative. In this context, we retrospectively evaluated all SF raw data fi les for the CoNS amplifi cation curve and its CP. The CoNS curve with CP >20 was present in 15 out of 44 (34%) SF staphylococci-negative with a lowest CP of 21.4, supporting the necessity for the discrimination threshold in the SF evaluation algorithm for commensal bacteria.