Skip to main content
Fig. 5 | Critical Care

Fig. 5

From: Fibroblast growth factor 21 attenuates ventilator-induced lung injury by inhibiting the NLRP3/caspase-1/GSDMD pyroptotic pathway

Fig. 5

FGF21 treatment ameliorates MS-induced injury in a cell model. A. FGFR1 expression in mouse liver, lung, and primary lung microvascular endothelial cells; B. Cell viability assessment by CCK8 assay (N = 6); C. Relative LDH level in cell culture supernatant (N = 6); D. Detection of reactive oxygen species by dichlorodihydrofluorescein probe in situ (N = 3, scale bar = 100 μm); E. Trolox-equivalent antioxidant capacity in cells (N = 6); F. Superoxide dismutase activity in cells (N = 6). G. Adenosine triphosphate level in cells (N = 6); H. Mitochondrial membrane potential and cell death staining (N = 6, scale bar = 100 μm); ns, no significance, *P < 0.05, **P < 0.01 vs. Control; #P < 0.05, ##P < 0.01 vs. PBS; FGFR1, fibroblast growth factor receptor 1; LMVESs, lung microvascular endothelial cells; MS, mechanical stretch; LDH, lactate dehydrogenase; DCF, dichlorodihydrofluorescein; TEAC, Trolox-equivalent antioxidant capacity; SOD, Superoxide dismutase

Back to article page