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Fig. 1 | Critical Care

Fig. 1

From: Real-time monitoring of air pathogens in the ICU with biosensors and robots

Fig. 1

A real-time air microbes monitoring system for nosocomial infection management in ICU. A. An automated guided vehicle (AGV) equipped with real-time air microbes monitoring system navigated in the ICU to collect air samples and detect the pathogenic microorganism. B. The real-time air microbes monitoring system consisted of an AGV, an air sample collector, and a microbes detecting system. The AGV had a Mecanum wheel chassis, which enable it to navigate freely in narrow spaces. The air sample collector had an air inlet, airway, air outlet, pump, and replaceable sampling tubes. C. The airborne pathogenic microorganism detecting system included disposable reaction wells, freeze-dried biosensors (RPA for target sequence amplification, CRISPR-Cas system for high sensitivity detection of the target sequence), fluorescence detecting system (optical lens, fiber, COM detector), and wireless for connecting the Internet of things (IoT). D. Target sequences were released by lysis of the pathogenic microorganisms in air samples, amplified by RPA, and were then detected by the CRISPR-Cas system which cuts the reporter RNA and releases fluorescence with the presence of the target sequence

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