Fig. 4

IL-6 inhibition significantly reduces amygdalar or hippocampal CC3 expression. A PMNs in BALF after VILI were significantly different from SB regardless of the three intervention groups (ANOVA, F = 9.861, dF = 21, p = 0.0005). There are no significant differences in oxygen saturations between the three VILI intervention groups; VILI + Saline, VILI + α-IL-6, and VILI + α-IL-6-receptor antibody. B–C Amygdalar (ANOVA, F = 8.738, dF = 21, p = 0.0009) and hippocampal (ANOVA, F = 9.806, dF = 21, p = 0.0005) CC3 expressions are significantly increased in the VILI + Saline group compared to SB control, but significantly reduced in both IL-6 inhibited groups. D–E IL-6 (ANOVA, F = 5.160, dF = 21, p = 0.0095) and TNF-α (ANOVA, F = 5.655, dF = 21, p = 0.0066) are significantly increased in the VILI + Saline group compared to SB control, but significantly reduced in both VILI + IL-6 inhibited groups. F There is no significant difference in percent area of amygdalar IL-1β between all groups. G–I Similar to amygdalar cytokines, IL-6 (ANOVA, F = 8.658, dF = 21, p = 0.0009) and TNF-α (ANOVA, F = 4.210, dF = 21, p = 0.0202) are significantly increased in the VILI + Saline group compared to SB control and significantly reduced in both VILI + IL-6 inhibiter groups; however, there is no difference in the percent area of IL-1β between all groups