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Fig. 1 | Critical Care

Fig. 1

From: Recombinant ACE2 protein protects against acute lung injury induced by SARS-CoV-2 spike RBD protein

Fig. 1

SARS-CoV-2 spike RBD protein enhanced the severity of LPS-induced ALI. A The study design for the current study. Male C57BL/6J mice were intratracheally administrated with saline or LPS (0.5 mg/kg). SARS-CoV-2 spike RBD-Fc (5.5 nmol/kg) or equivalent Control-Fc was intraperitoneally injected three times (at 30 min before and at 1 and 2 h after) during LPS treatment. The lung tissues were obtained 3 d after LPS treatment for further assessment of the severity of ALI. B. The representative images of H&E staining of the lung tissues. The upper panel, 100×, scale bar = 100 μm; the bottom panel, 400×, scale bar = 100 μm. C. The lung injury score was calculated according to the results of H&E staining. (40 fields for each sample were determined.) D. The wet-to-dry weight ratio of the lung tissues was determined (n = 5 for each group). E The cell counts in the BALF were determined (n = 6 for each group). F The protein concentrations of the BALF were determined by BCA (n = 6 for each group). Data are shown as mean ± SD, *P < 0.05, **P < 0.01, and N.S. indicates not significant

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