Fig. 4

Biological pathway analysis and immune cell-type deconvolution of transcriptional subtypes. a–d Ingenuity Pathway Analysis of canonical signaling gene sets differentially enriched across transcriptional subtypes based on log-fold change ≥|1| and Benjamini–Hochberg adjusted p-value ≤ 0.01; Z-score indicates up- versus down-regulation of signaling gene sets in subtype 2 vs. 1 (N = 128). e Relative quantities of immune cell-types inferred across subtypes based on ImmQuant digital cell quantification deconvolution; red shading indicates a higher inferred quantity of cell-type in subtype 2 vs. 1 based on log-fold change; blue shading indicates a lower inferred quantity of cell-type in subtype 2 vs. 1 based on log-fold change (N = 128). f Hematopoietic lineage plot with relative quantities of immune cell-types inferred across subtypes based on ImmQuant digital cell quantification deconvolution (N = 128); intensity of red shading indicates a higher inferred quantity of cell-type in subtype 2 vs. 1 based on log-fold change; intensity of blue shading indicates a lower inferred quantity of cell-type in subtype 2 vs. 1 based on log-fold change