Fig. 3

The fate of supplemented 3-hydroxybutyrate as cholesterogenic or TCA cycle substrate. a Tracer conversion overview. For each individual mouse, the fractional contribution of each labeled metabolite (indicating the fraction labeled metabolite of the total amount of metabolite) in each tissue was corrected for the fractional contribution of 3HB in plasma. b Tissue-specific uptake of ¹³C-labeled 3-hydroxybutyrate. c Overall fractional contribution of metabolites. Tissue-specific fractional labeling of d mevalonate, e citrate and f fumarate [White: healthy controls n = 7, light blue: septic day 2 mice n = 7, dark blue: septic day 5 mice n = 7]. Data in boxplots represent median and interquartile range; dots represent outliers. §p ≤ 0.05 §§p ≤ 0.01 §§§p ≤ 0.001 between healthy controls and septic mice, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 between day 2 and day 5 of sepsis