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Table 3 BBB correlated proteins improved outcome prediction independently following severe TBI

From: Fluid proteomics of CSF and serum reveal important neuroinflammatory proteins in blood–brain barrier disruption and outcome prediction following severe traumatic brain injury: a prospective, observational study

Protein, antibody

Compartment

Highest tissue enrichment

Protein size [kDa]

Coefficient

ΔR2

adjusted p value

QA subgroup analysis

STMN4 HPA078407

CSF

cns

19.4–30.4a

− 0.00505

0.121

0.04548

No

C5 HPA075945

CSF

Liver/gallbladder

188.3

− 0.00095

0.106

0.04548

No

GPR26 HPA062736

CSF

cns

37.6

− 0.00684

0.099

0.04548

No

CFB HPA001817

Serum

Liver/gallbladder

85.5

0.00098

0.092

0.04548

Yes

FCN1 HPA001295

Serum

Blood

35.1

0.00303

0.082

0.04548

Yes

C9 HPA070709

CSF

Liver/gallbladder

63.2

− 0.00123

0.074

0.04548

Yes

IL6 HPA064428

Serum

Adipose/soft tissue

13.9–23.7a

0.00185

0.071

0.04548

Yes

  1. All proteins that comprised the intersect between CSF-altered proteins and CSF cluster-derived proteins or serum-altered proteins and CSF cluster derived proteins were used for outcome analysis. Outcome prediction was conducted by univariable followed by multivariable proportional odds regression analysis where GOS was used as dependent variable and the protein level as independent variable. The IMPACT variables were used as covariates. Here we show the n = 3 proteins that conferred the highest ΔNagelkerke’s pseudo-R2 (decimal number) in CSF (row 1–3), in serum (row 4, 5, 7), and upon specific outcome analysis for proteins significantly different between patients with intact and disrupted BBB (row 4–6). Proteins that were significantly different between disrupted and intact BBB (CFB, FCN1, C9, IL-6) were subjected to a sub-group analysis (“QA subgroup analysis” column), for which adjusted p values are described in Additional file 3: Table S7. Protein sizes were derived from the Human Protein Atlas for the specific protein splice variant represented by the Human Protein Atlas antibody
  2. BBB blood–brain barrier injury, CNS central nervous system, Coeff. regression coefficient, CSF cerebrospinal fluid, Da Dalton, GOS Glasgow Outcome Score, IMPACT International Mission for Prognosis and Analysis of Clinical Trials in TBI, TBI traumatic brain injury, QA albumin quotient. All full protein names are listed in Additional file 3: Table S1
  3. aProtein size interval provided as Human Protein Atlas antibody is not specified for protein splice variant
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Critical Care

ISSN: 1364-8535