Fig. 10

Effects of inactive and active VD3 after the onset of CLP-induced polymicrobial sepsis. a All mice underwent CLP surgery and were administered with water or inactive VD3 (50 μg/kg) for one time by oral gavage immediately after CLP (n = 8 per group). Mice treated with inactive VD3 even had a higher 7-day mortality and b higher day-1 MSS score. CLP-induced polymicrobial sepsis resulted in hepatic damage. CLP-operated mice demonstrated increased c AST and ALT levels, d decreased CYP2R1 and CYP27A1 mRNA levels, and e reduced serum VD3 levels (intermediate plus active forms) (n = 4–5 per group). For active VD3 treatment, all mice underwent CLP surgery and were administered with water or active VD3 (50 μg/kg) for 7 days by intraperitoneal injection (n = 11 per group). Mice treated with active VD3 had better outcomes in terms of f 7-day mortality, g day 2 and day 3 MSS score as well as d higher serum vitamin D3 levels (intermediate plus active forms).*P < 0.05; **P < 0.01; ***P < 0.001