Fig. 3
From: Venoarterial extracorporeal membrane oxygenation induces early immune alterations
VA-ECMO induced MDSC expansion and T-cell dysfunction. a Total lymphocytes were determined by blood count formula. b Proportion of apoptotic T-cells and their subpopulations, CD4pos and CD8pos, were determined at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 6 Control (Ctrl) group, n = 6 VA-ECMO group) by flow cytometry with an annexinV binding assay (n = 6). Results expressed as percentage of AnnexinVpos Dapineg cells. c Fresh PBMC obtained at admission or VA-ECMO initiation (D0) and 24 h after (D1) were stimulated with anti-CD3/anti-CD28 monoclonal antibodies after CFSE labelling. The proportion of CD4pos (two left graphics) and CD8pos (two right graphics) proliferated T cells were determined by flow cytometry (n = 9 Ctrl group, n = 7 ECMO group). d Percentage of granulocytic (G)-MDSC among PBMCs was determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). Peripheral monocytic (M)-MDSC counts were determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group)