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Fig. 2 | Critical Care

Fig. 2

From: Venoarterial extracorporeal membrane oxygenation induces early immune alterations

Fig. 2

VA-ECMO induced changes in antigen presenting cell phenotype. a Peripheral monocyte counts were determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). b Monocyte HLA-DR expression was measured by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). HLA-DR expression has been represented as ratio of mean fluorescence intensity ratio (rrMFI) defined as the ratio of rMFI between D0 and D1 or D4. c Peripheral dendritic cell (DC) counts were determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). d Dendritic cells HLA-DR expression was measured by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group). HLA-DR expression has been represented as ratio of mean fluorescence intensity ratio (rrMFI) defined as the ratio of rMFI between D0 and D1 or D4. (n = 8 Control (Ctrl) group, n = 9 ECMO group). E. Peripheral dendritic cell (DC) subsets counts (plasmacytoid DC (pDC) and myeloid DC (mDC CD1cpos and mDC CD141pos)) were determined by flow cytometry at admission or VA-ECMO initiation (D0), 24 h and 4 days (D1 and D4) (n = 8 Control (Ctrl) group, n = 9 VA-ECMO group)

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